TU Dresden


A molecular view of microtubule dynamic instability and it’s regulation by mitotic kinesins

CMCB Life Sciences Seminar

Datum:26/09/2019, 16:00 - 17:00
Sprecher: Prof. William O. Hancock, Pennsylvania State University, Department of Biomedical Engineering
Ort: CRTD, ground floor, auditorium left
Gastgeber: Prof. Stefan Diez (B CUBE)

Despite the importance of microtubules in cell division and cell migration, and the fact that they are targets of a number of anticancer drugs, the molecular mechanism of microtubule dynamic instability is not understood. Using Interferometric Scattering Microscopy to visualize gold nanoparticle-labeled tubulin incorporating into growing microtubules, we are able to directly measure the on- and off-rates for tubulin incorporation into the microtubule lattice, and thus provide new quantitative constraints for models of dynamic instability.  We are also investigating the regulation of dynamic instability by mitotic kinesins from the kinesin-5 family.  We find that kinesin-5 enhances microtubule polymerization by stabilizing a straight conformation of tubulin.  These high-resolution studies will be discussed in the context of the numerous roles of microtubules in cells.

5 most important publications
Direct observation of individual tubulin dimers binding to growing microtubules. Mickolajczyk, K. J., E. A. Geyer, T. Kim, L. M. Rice, and W. O. Hancock. 2019. Proc Natl Acad Sci U S A 116:7314-7322.

Kinesin-5 Promotes Microtubule Nucleation and Assembly by Stabilizing a Lattice-Competent Conformation of Tubulin.Chen, G. Y., J. M. Cleary, A. B. Asenjo, Y. Chen, J. A. Mascaro, D. F. J. Arginteanu, H. Sosa, and W. O. Hancock. 2019. Curr Biol 29:2259-2269 e2254.

Kinetics of nucleotide-dependent structural transitions in the kinesin-1 hydrolysis cycle. 2015. Mickolajczyk, K. J., N. C. Deffenbaugh, J. Ortega Arroyo, J. Andrecka, P. Kukura, and W. O. Hancock. Proc. Natl. Acad. Sci., 112:E7186-7193.  PMID: 26676576

Kinesin-5 is a microtubule polymerase. 2015. Chen, Y., and W.O. Hancock. Nature Communications 6:8160. doi:10.1038/ncomms9160.  PMID: 26437877

Bidirectional cargo transport: moving beyond tug of war. Hancock W.O. 2014. Nat Rev Mol Cell Biol. 15(9):615-28.  PMID: 25118718

Everybody is very welcome!