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Finding your place: transcription factors as sensors and modifiers of chromatin

CMCB Life Sciences Seminar

Datum:18/06/2020, 13:00 - 14:00
Sprecher: Dirk Schubeler, Friedrich Miescher Institute for Biomedical Research
Ort: CRTD, ground floor, auditorium left
Gastgeber: Mareike Albert

Link to the conference -  Zoom Meeting ID:  972 3820 1359, Password: 647556

Abstract:

Transcription factors only bind a minority of their motifs in large mammalian genomes. One potential explanation is that many motifs are not accessible for binding due to the action of chromatin and DNA methylation. We are using mammalian stem cell models to understand this important interplay between gene regulation, chromatin structure and DNA methylation. We study the dynamics of the epigenome and test regulatory models in cellular models by genetic perturbation and genome editing approaches.

I will discuss our recent efforts in understanding how the sensitivity to DNA methylation can limit transcription factor binding in the context of the cell and how TFs rely on specific chromatin remodelers for access to their binding sites.

5 most important publications:

Darko Barisic*, Michael Stadler*, Mario Iurlaro* and Dirk Schübeler. (2019) Mammalian ISWI and SWI/SNF selectively mediate binding of distinct groups of transcription factors, Nature 569(7754):136-140   

Domcke, S.*, Bardet, A. F.*, Ginno, P., Hartl, D., Burger, L., and Schübeler, D. (2015) Competition between DNA methylation and transcription factors determines binding of NRF1. (*joint 1st), Nature, 528 (7583); 575-579.   

Tuncay Baubec, Robert Ivanek, Florian Lienert and Dirk Schübeler. (2013) Methylation-dependent and -independent genomic targeting principles of the MBD protein family, Cell 153, 480–492   

Michael B. Stadler*, Rabih Murr*, Lukas Burger*, Robert Ivanek, Florian Lienert, Anne Schöler, Christiane Wirbelauer, Erik van Nimwegen, Edward J. Oakeley, Dimos Gaidatzis, Vijay K. Tiwari and Dirk Schübeler. (2011) DNA binding factors shape the mouse methylome at distal regulatory regions, Nature, 480, 490-495

Weber, M., Hellmann, I., Stadler, M., Ramos, L., Pääbo, S., Rebhan, M. and Schübeler, D. (2007). Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome, Nature Genetics, 39, 457-466

Everybody is very welcome!