TU Dresden


Active deformation of the cell membrane through actin assembly, and how we may infer nucleus deformation

CMCB Life Sciences Seminar

Date:28/05/2020, 13:00 - 14:00
Speaker: Cécile Sykes, Institut Curie/ PSL/ Sorbonne Université
Location: Zoom Video Conferencing
Host: Elisabeth Fischer-Friedrich

Link to the conference -  Zoom Meeting ID: 934 2705 8190, Password: 175528


We conceive stripped-down experimental systems that reproduce cellular behaviours in simplified conditions: cytoskeleton dynamics are reproduced on liposome membranes. By changing experimentally membrane tension and the structural details of the cytoskeleton architecture, we draw a phase diagram where inward or outward deformations are favoured. We observe buckling and wrinkling under osmotic deflation, thereby confirming network elastic properties. Our results illustrate the generic mechanism of buckling and wrinkling found in various systems spanning from pollen grains to the development of the gut or the brain.

Inspired by actin forces exerted on membranes and organelles, we address now how the nucleus, which is the most rigid cell organelle, is deformed by the actin cytoskeleton during cell translocation.

5 most important publications:

C. Simon, V. Caorsi, C. Campillo, C. Sykes, "Interplay between membrane tension and the actin cytoskeleton determines shape changes",Physical Biology, 5(6):065004, doi: 10.1088/1478-3975/aad1ab. (2018)

A. Allard, M. Bouzid, T. Betz, C. Simon, M. Abou-Ghali, J. Lemière, F. Valentino, J. Manzi, F. Brochard-Wyart, K. Guevorkian, J. Plastino, M. Lenz, C. Campillo*, C. Sykes*, "Actin modulates shape and mechanics of tubular membranes” bioRxiv 712505; doi:, and, Science advances in press

C. Simon, R. Kusters, V. Caorsi, A. Allard, M. Abou-Ghali, J. Manzi, A. Di Cicco, D. Lévy, M. Lenz, J.-F. Joanny, C. Campillo, J. Plastino, P. Sens, C. Sykes " Actin dynamics drive cell-like membrane deformation " Nature Physics, (2019)

R. Kusters, C. Simon, R. Lopes Dos Santos, V. Caorsi, Sangsong Wu, J.-F. Joanny, P. Sens, C. Sykes, “Actin shells control buckling and wrinkling of biomembranes” Soft Matter, 15, 9647 – 9653 doi: 10.1039/c9sm01902b (2019)

P.M. Davidson, A. Battistella, T. Déjardin, T. Betz, J. Plastino, N. Borghi, B. Cadot, C. Sykes “Actin accumulates nesprin-2 at the front of the nucleus during confined cell migration” bioRxiv 713982; doi:, EMBO Reports, in press