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Visualising DNA repair and mutagenesis at the single-molecule level in bacteria

CMCB Life Sciences Seminar

Date:25/06/2020, 13:00 - 14:00
Speaker: Stephan Uphoff, University of Oxford
Location: Online
Host: Michael Schlierf

Link to the conference -  Zoom Meeting ID: 873 3947 3679, Password: 245197

Abstract:

DNA repair and mutagenesis define the balance between genome stability and plasticity. To understand the molecular mechanisms and their regulation, our group develops microscopy methods to visualise DNA repair proteins at a single-molecule level and to detect mutations in real-time within living bacterial cells. These tools provide an unprecedented direct view into the molecular processes that guard DNA stability or promote mutagenesis when bacteria experience stress conditions.

5 most important publications:

Lawarée E, Jankevicius G, Cooper C, Ahel I, Uphoff S, Tang C., DNA ADP-Ribosylation Stalls Replication and Is Reversed by RecF-Mediated Homologous Recombination and Nucleotide Excision Repair, Cell Reports 30, 1373-1384 (2020)

Uphoff S, Real-time dynamics of mutagenesis reveal the chronology of DNA repair and damage tolerance responses in single cells, PNAS 115, E6516-E6525 (2018)

Stracy M, Jaciuk M, Uphoff S, Kapanidis AN, Nowotny M, Sherratt DJ, Zawadzki P., Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli, Nature Communications, 7, 12568 (2016)

Uphoff S, Lord ND, Potvin-Trottier L, Okumus B, Sherratt DJ, Paulsson J., Stochastic activation of a DNA damage response causes cell-to-cell mutation rate variation, Science, 27290, 1094-1097 (2016)

Uphoff S, Reyes-Lamothe R, Garza de Leon F, Sherratt DJ, Kapanidis AN, Single-molecule DNA repair in live bacteria, PNAS , 110(20), 8063-8068 (2013)

Everybody is very welcome!