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Neuronal Cell Types and Circuit Engineering - Volker Busskamp

In order to understand how parts of the human brain, especially the retina, function in health and disease, our research focus is to reverse engineer functional human neuronal circuits from scratch combining neuroscience with stem cell research and bioengineering. Our goal is to generate the basic parts, namely electrically active neurons, from adult human induced stem cells in high yields.

Previous and current research

Previously our focus was to study the human and mouse retina in health and disease. In a blinding disease called Retinitis pigmentosa, the goal was to repair non-functional cone photoreceptors using optogenetics, specifically by expressing microbial Halorhodopsins, to restore visual function in mice. We further translated this approach to post mortem human retinas (Busskamp et al. 2010), which forms the basis for on-going clinical trials. more

Future projects and goals

In order to understand how parts of the human brain function in health and disease, our major aim is to reverse engineer functional human neuronal circuits from scratch combining neuroscience with stem cell research and bioengineering. We plan to generate the basic parts, namely electrically active neurons, from adult human stem cells. Next, we need to understand and control the biology to connect these cells in a reproducible way into defined functional neuronal circuits in vitro. more

Contact

Group Leader

Dr. Volker Busskamp

volker.busskamp[at]tu-dresden.de


Assistant

Anne Gerber

Phone: +49 (0)351 458 82354

Telefax: +49 (0)351 458 82059

Email: anne-kathrin.gerber[at]tu-dresden.de

 

 

 

Selected publications

Kutsche L, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp V. “Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis”, Cell Systems 2018 Oct 3 (advanced online publication).

 

Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, Busskamp V. "On-demand optogenetic activation of human stem-cell-derived neurons", Scientific Reports 2017 Oct 31;7(1):14450. 

 

Busskamp V, Lewis NE, Guye P, Ng AHM, Shipman SL, Byrne SE, Murn J, Sanjana NE, Li S, Li Y, Stadler M, Weiss R, Church GM. “Rapid neurogenesis through transcriptional activation in human stem cells”, Molecular Systems Biology 2014 Nov 17;10(11):760

 

Busskamp V, Krol J, Nelidova D, Daum J, Szikra T, Tsuda B, Jüttner J, Farrow K, Gross Scherf B, Patino Alvarez CP, Genoud C, Sothilingam V, Tanimoto N, Stadler M, Seeliger M, Stoffel M, Filipowicz M, Roska. “MicroRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function”, Neuron 2014 Aug 6;83(3):586-600

 

Busskamp V, Duebel J, Balya D, Fradot M, Viney TJ, Siegert S, Groner AC, Cabuy E, Forster V, Seeliger M, Biel M, Humphries P, Paques M, Mohand-Said S, Trono D, Deisseroth K, Sahel JA, Picaud S, Roska B. ”Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis pigmentosa”, Science. 23 July 2010: Vol. 329. no. 5990, pp. 413 – 417.

 

All publications can be found on PubMed and downloaded via ResearchGate.

 

Group members

(c) Dr. Sacha Hanig, TU Dresden

Group leader

Funding

 

Lab activities and News

2018/08/23: Our miR-124 paper is online at Cell Systems!

2018/09/24: Evelyn successfully defended her PhD thesis. Congratulations!

2018/09/10: Evelyn's 'Methods in Molecular Biology' paper is in press.

2018/09/07: Marta, Jenna, Giovanni and Eduardo won the CRTD Summer conference "Bowle Contest". Cheers to "Memory and No-Memory".

2018/09/03: Our joint grant application with the Stieger lab receives DFG funding.

2018/07/24: Our joint grant application with the Czarske lab receives DFG funding.

2018/07/19: Our joint paper with the Czarske lab is published. Congrats to Felix and Simon.

2018/05/01: Marta presented our collaborative work with the Karl lab at ARVO 2018 (Honolulu, HI).

 

 

 

Joining the lab

In general, due to the high number of applications for intern, student, PhD and Postdoc positions we are unfortunately unable to answer all requests. If your application has caught our attention, we will get back to you. Future job offers and PhD student positions will be advertised.

***We have a lab technician position to be filled.***

Upcoming lab presentations

2018/11/13: BioForum 2018, Shanghai, China

2018/11/22: Smart Systems (13th IPF Colloquium), Dresden, Germany

2019/04/28: ARVO Annual Meeting, Vancouver, Canada

 

 

Resources

Our published plasmids can be requested from Addgene.