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7. Developing site-specific recombinase technology for adult zebrafish brain

We demonstrated that using transposon-mediated transgenesis, the site-specific recombinase Cre is highly efficient and spatiotemporally controllable in zebrafish. Our work provides the opportunity to develop recombinase-mediated techniques for genomic manipulations including conditional knockout alleles, temporal and spatial overexpression studies and bona fide lineage tracing.

Scientists involved: Dr. Stefan Hans, Peggy Jungke

Related publications:
Hans, S., Kaslin, J., Freudenreich, D., and Brand, M. (2009). Temporally-controlled Site-specific Recombination in Zebrafish. PLoS ONE. 2009;4(2):e4640.

Hans, S., Freudenreich, D., Geffarth, M., Kaslin, J., Machate, A. and  Michael Brand (2011). Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish. Developmental Dynamics 240(1):108-115.

Jungke P, Hans S, Brand M.  (2013) The Zebrafish CreZoo: An Easy-to-Handle Database for Novel CreER(T2)-Driver Lines. Zebrafish.

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