English
Deutsch
TU Dresden

search

Neuronal Cell Types and Circuit Engineering - Volker Busskamp

In order to understand how parts of the human brain, especially the retina, function in health and disease, our research focus is to reverse engineer functional human neuronal circuits from scratch combining neuroscience with stem cell research and bioengineering. Our goal is to generate the basic parts, namely electrically active neurons, from adult human induced stem cells in high yields.

Previous and current research

Previously our focus was to study the human and mouse retina in health and disease. In a blinding disease called Retinitis pigmentosa, the goal was to repair non-functional cone photoreceptors using optogenetics, specifically by expressing microbial Halorhodopsins, to restore visual function in mice. We further translated this approach to post mortem human retinas (Busskamp et al. 2010), which forms the basis for on-going clinical trials. more

Future projects and goals

In order to understand how parts of the human brain function in health and disease, our major aim is to reverse engineer functional human neuronal circuits from scratch combining neuroscience with stem cell research and bioengineering. We plan to generate the basic parts, namely electrically active neurons, from adult human stem cells. Next, we need to understand and control the biology to connect these cells in a reproducible way into defined functional neuronal circuits in vitro. more

Contact

Group Leader

Dr. Volker Busskamp

volker.busskamp[at]tu-dresden.de


Assistant

Anne Gerber

Phone: +49 (0)351 458 82354

Telefax: +49 (0)351 458 82059

Email: anne-kathrin.gerber[at]tu-dresden.de

 

 

 

Selected publications

Kutsche L, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp V. “Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis”, Cell Systems 2018 Oct 3 (advanced online publication).

 

Busskamp V, Lewis NE, Guye P, Ng AHM, Shipman SL, Byrne SE, Murn J, Sanjana NE, Li S, Li Y, Stadler M, Weiss R, Church GM. “Rapid neurogenesis through transcriptional activation in human stem cells”, Molecular Systems Biology 2014 Nov 17;10(11):760

 

Busskamp V, Krol J, Nelidova D, Daum J, Szikra T, Tsuda B, Jüttner J, Farrow K, Gross Scherf B, Patino Alvarez CP, Genoud C, Sothilingam V, Tanimoto N, Stadler M, Seeliger M, Stoffel M, Filipowicz M, Roska. “MicroRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function”, Neuron 2014 Aug 6;83(3):586-600

 

Busskamp V, Duebel J, Balya D, Fradot M, Viney TJ, Siegert S, Groner AC, Cabuy E, Forster V, Seeliger M, Biel M, Humphries P, Paques M, Mohand-Said S, Trono D, Deisseroth K, Sahel JA, Picaud S, Roska B. ”Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis pigmentosa”, Science. 23 July 2010: Vol. 329. no. 5990, pp. 413 – 417.

 

All publications can be found on PubMed and downloaded via ResearchGate.

 

Group members

(c) Dr. Sacha Hanig, TU Dresden

Group leader

Resources

Our published plasmids can be requested from Addgene.

 

 

 

Funding

Lab activities and News

2019/05/15: Congrats to Marta, Eduardo and Stefanie Wohl, our review paper "Retinal miRNA functions in health and disease" is accepted in "Genes".

2019/04/26: iNGN cells differentiate on hydrogel. We are happy having contributed to the Minev's group article "Highly Conductive, Stretchable, and Cell-Adhesive Hydrogel by Nanoclay Doping".

2019/03/25: Evelyn's method paper "Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders" is now published in Methods in Molecular Biology.

2019/03/08: Eduardo's review article "Challenges in miRNAs’ targetome prediction and validation" is in press. Stay tuned.

2019/01/14: Our Physics of Life (PoL) Starting Grant application in collaboration with Carsten Werner's team was successful.

2018/08/23: Our miR-124 paper is online at Cell Systems!

 

 

 

 

Upcoming lab presentations

2019/08/30: ISGEDR Meeting, Giessen, Germany

2019/09/02: Ribbon Synapses Symposium (RSS) 2019, Goettingen, Germany

2019/09/20: VII. International DOG-Symposium on AMD, Baden-Baden, Germany

 

 

 

 

 

Joining the lab

The Busskamp lab will relocate to the University of Bonn. Stay tuned for future job offers. Due to our relocation, unfortunately, we won’t offer any longer intern or rotation projects at CRTD.