Deutsch
TU Dresden

search

Neuronal Cell Types and Circuit Engineering - Volker Busskamp

In order to understand how parts of the human brain, especially the retina, function in health and disease, our research focus is to reverse engineer functional human neuronal circuits from scratch combining neuroscience with stem cell research and bioengineering. Our goal is to generate the basic parts, namely electrically active neurons, from adult human induced stem cells in high yields.

Previous and current research

Previously our focus was to study the human and mouse retina in health and disease. In a blinding disease called Retinitis pigmentosa, the goal was to repair non-functional cone photoreceptors using optogenetics, specifically by expressing microbial Halorhodopsins, to restore visual function in mice. We further translated this approach to post mortem human retinas (Busskamp et al. 2010), which forms the basis for on-going clinical trials. more

Future projects and goals

In order to understand how parts of the human brain function in health and disease, our major aim is to reverse engineer functional human neuronal circuits from scratch combining neuroscience with stem cell research and bioengineering. We plan to generate the basic parts, namely electrically active neurons, from adult human stem cells. Next, we need to understand and control the biology to connect these cells in a reproducible way into defined functional neuronal circuits in vitro. more

Contact

Group Leader

Dr. Volker Busskamp

volker.busskamp[at]tu-dresden.de


Assistant

Eike Lau

Phone: +49 (0)351 458 82354

Telefax: +49 (0)351 458 82059

Email: eike.lau[at]tu-dresden.de

 

 

 

Selected publications

Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, Busskamp V. "On-demand optogenetic activation of human stem-cell-derived neurons", Scientific Reports. 2017 Oct 31;7(1):14450. 

 

Busskamp V, Lewis NE, Guye P, Ng AHM, Shipman SL, Byrne SE, Murn J, Sanjana NE, Li S, Li Y, Stadler M, Weiss R, Church GM. “Rapid neurogenesis through transcriptional activation in human stem cells”, Molecular Systems Biology 2014 Nov 17;10(11):760

 

Busskamp V, Krol J, Nelidova D, Daum J, Szikra T, Tsuda B, Jüttner J, Farrow K, Gross Scherf B, Patino Alvarez CP, Genoud C, Sothilingam V, Tanimoto N, Stadler M, Seeliger M, Stoffel M, Filipowicz M, Roska. “MicroRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function”, Neuron 2014 Aug 6;83(3):586-600

 

Busskamp V, Duebel J, Balya D, Fradot M, Viney TJ, Siegert S, Groner AC, Cabuy E, Forster V, Seeliger M, Biel M, Humphries P, Paques M, Mohand-Said S, Trono D, Deisseroth K, Sahel JA, Picaud S, Roska B. ”Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis pigmentosa”, Science. 23 July 2010: Vol. 329. no. 5990, pp. 413 – 417.

 

All publications can be found on PubMed and downloaded via ResearchGate.

 

Group members

(c) Dr. Sacha Hanig, TU Dresden

Group leader

Funding

 

Lab activities and News

2018/05/01: Marta presented our collaborative work with the Karl lab at ARVO 2018 (Honolulu, HI).

2018/04/06: Alex Ng successfully defended his PhD thesis about the TFome.

2018/03/13: We met the Jakobsson lab for a joint lab retreat at the Baltic Sea.

2018/02/09: Lisa presented her work on miR-124 regulation in human neurons at the CMCB seminar and we will host the Beer Hour together with the Minev lab.

2017/11/14: Lisa gave an exciting talk about miR-124 regulation in human neurons at the Society for Neuroscience Meeting in Washington DC.

2017/10/16: Our paper entitled "On-demand optogenetic activation of human stem-cell-derived neurons" is accepted. Congratulations to the lead authors Evelyn and Simon.

 

 

 

Joining the lab

In general, due to the high number of applications for intern, student, PhD and Postdoc positions we are unfortunately unable to answer all requests. If your application has caught our attention, we will get back to you. Future job offers and PhD student positions will be advertised.

Resources

Our published plasmids can be requested from Addgene.